Journal: MethodsX

Article Title: A simple judgment method for joint action of antibacterial agents on bacterial resistance

doi: 10.1016/j.mex.2022.101700

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Article Snippet: Setting E. coli MG1655 K-12 (purchased from Biovector Co., Ltd. (Bejing, China)) as the model bacteria, the dose-response relationship of single antibacterial agent to the growth of E. coli was determined using microplate toxicity analysis.

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Article Title: Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori : the Malate:Quinone Oxidoreductase

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: C. glutamicum was grown overnight on 2× tryptone-yeast extract medium ( 25 ) at 30°C with, in the case of the strains carrying the marker, 25 μg of kanamycin ml −1 . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristics Source or reference(s) Strains C. glutamicum ATCC 13032 Wild type 1 Δ mqo mqo deletion derivative of ATCC 13032 M. E. van der Rest, unpublished data H. pylori ATCC 49503 Wild type 12 E. coli DH5α supE44 ΔlacU169 (φ80d lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 25 Δ mqo mqo ( yojH ) deletion derivative of MC4100 4, M. E. van der Rest, unpublished data Plasmids pBluescript II SK Amp r lacZ 26 pEKEx1 Kan r lacl q tac 15 pHp-mqo pEKEx1 with H. pylori mqo gene (ORF HP0086) This paper Open in a separate window Bacterial strains and plasmids The minimal medium agar used in this study for growth of C. glutamicum will be described elsewhere (D. Molenaar, unpublished data).

Techniques: Plasmid Preparation

Journal:

Article Title: Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori : the Malate:Quinone Oxidoreductase

doi:

Figure Lengend Snippet: MQO activities measured by DCPIP reduction or oxygen consumption in E. coli and C. glutamicum Δmqo strains expressing ORF HP0086 from a plasmid

Article Snippet: C. glutamicum was grown overnight on 2× tryptone-yeast extract medium ( 25 ) at 30°C with, in the case of the strains carrying the marker, 25 μg of kanamycin ml −1 . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristics Source or reference(s) Strains C. glutamicum ATCC 13032 Wild type 1 Δ mqo mqo deletion derivative of ATCC 13032 M. E. van der Rest, unpublished data H. pylori ATCC 49503 Wild type 12 E. coli DH5α supE44 ΔlacU169 (φ80d lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 25 Δ mqo mqo ( yojH ) deletion derivative of MC4100 4, M. E. van der Rest, unpublished data Plasmids pBluescript II SK Amp r lacZ 26 pEKEx1 Kan r lacl q tac 15 pHp-mqo pEKEx1 with H. pylori mqo gene (ORF HP0086) This paper Open in a separate window Bacterial strains and plasmids The minimal medium agar used in this study for growth of C. glutamicum will be described elsewhere (D. Molenaar, unpublished data).

Techniques: Expressing, Plasmid Preparation, Activity Assay

Journal: Applied Microbiology and Biotechnology

Article Title: High-throughput screening of environmental polysaccharide-degrading bacteria using biomass containment and complex insoluble substrates

doi: 10.1007/s00253-020-10469-3

Figure Lengend Snippet: Using mBCDs to screen environmental isolates capacity to degrade insoluble substrates . Either ( a ) corn stover or ( b ) glutinous rice was used as the only source of carbon. The tested strains were Cellvibrio japonicus ( Cj WT ), Cellvibrio japonicus Δ gsp ( Cj Δ gsp), Escherichia coli K-12 (K-12), Arthrobacter nicotianae (WW33), Klebsiella oxytoca (WW55), Enterobacter spp. (WW64), and Bacillus firmus (LZ66). All growth analysis experiments were done in biological triplicate at 30 °C with high levels of aeration. Error bars indicate standard deviation

Article Snippet: Cellvibrio japonicus Ueda107 (NCIMB 10463) and E. coli K-12 (NCIMB 10218) were obtained from the National Collections of Industrial, Marine, and Food Bacteria (Aberdeen, Scotland, UK).

Techniques: Standard Deviation